The Bioactive Components of Cascara Kombucha and Their Potential Use as a Functional Beverage
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This study aims to analyze the antimicrobial activity of cascara kombucha and evaluate its potential as a functional beverage through in vivo experiments using male white rats (Rattus norvegicus). The antimicrobial activity test utilized the kombucha derived from three types of cascara: arabica (J1), robusta (J2), and liberica (J3), which fermented using 10% of sugar for 10 and 17 days. The antimicrobial activity was tested against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 29213 in vitro using the disk diffusion method (Kirby-Bauer test). Black tea kombucha served as the control. The safety of cascara kombucha was evaluated by aspartate aminotransferase (AST), alanine aminotransferase (ALT), and total bilirubin levels in rats' blood. The data were analyzed using a T-test with RStudio software. The results indicated that cascara kombucha from arabica and liberica effectively inhibited the growth of E. coli and S. aureus. The administration of 1.8 ml of arabica cascara kombucha had no significant effect (P > 0.05) on the liver function based on the AST, ALT, and total bilirubin levels. The in vivo test demonstrated that cascara kombucha is safe for mammalian consumption. Overall, the findings pointed to the conclusion that cascara kombucha is an effective antimicrobial agent and has functional values without causing adverse effects on the liver function of male white rats.
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